Abstract- Flower image retrieval plays important role in automatic species recognition. In this paper we use segmentation based method on color clustering and domain knowledge two extract flower regions. Color moments of flower region are used to characterize the colour feature. In characterizing shape feature, we use Centroid color distance (CCD) and angle code histogram (ACH). Experimental results showed that clustering based flower region extraction method can produce good accuracy. Flower retrieval results in 16 species of flower images where yyy species gave the maximum 89% accuracy.