The objective of this research is to study and develop the tracking patient medical record system using radio frequency identification. first, documents and research paper related to tracking patient medical records and RFID were reviewed. then, the system was devoloped in the form of windows application by using agile technique. tools used for developing system included visual basic. net 2005, which connect to RFID, and used SQL server 2005 for database. the system was evaluated by survey 31 users. the result showed that users were satisfied with the system at a high level. the average satisfaction (X) was 3.89 and standard deviation (SD) was 0.64. this meand that the system can be used properly in naresuan university hospital.