Crude oil degradation has been carried out using biopile reactor in total petroleum hydrocarbon (THP) concentration of 5%, 10% and 15%. The thermohilic microorganisms used from isolation, result and identification are Aeromonas salmonicida, Bacillus pantothenticus, and stenotrophomonas maltophilia. The biodegradation of biopile reactor is done by various concentrations of TPH, total plate count (TPC), and volatile suspended solid (VSS) per day for 30 days. The biodegradation of kinetic parameter calculated consists of, m,y,yt,yobs,kd,ks from tPH concentration decisions. and TPC and VSS in very microorganism with t (observation time) of 0 hour to 168 hours. The crude oil separation efficiency in abiople reator shows that the largest separation occurs in a starting TPH concentration of 15%, which becomes 61.8% later on. followed by a starting TPH concentration of 10% and 5%, which is as much as 61% and 48.4%.